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Characterization of growth hormone releasing factor analog expression in Saccharomyces cerevisiae

โœ Scribed by CRAIG, WILLIAM S. ;WONDRACK, LILLIAN ;SIEGEL, ROBERT ;PATTHI, SARASWATHI ;DAVIS, GENEVA R. ;VELICELEBI, GONUL ;MOWLES, THOMAS F. ;THILL, GREGORY P.


Book ID
115099260
Publisher
Wiley (Blackwell Publishing)
Year
2009
Tongue
English
Weight
811 KB
Volume
38
Category
Article
ISSN
0367-8377

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Expression kinetics of the human Epidermal Growth Factor (hEGF) from the a-factor prepro region in a 2-pm based plasmid was studied in Saccharomyces cerevisiae. Production o f hEGF was highly medium dependent as a chemically defined, nonenriched media had a significantly lower yield than did enriche