Characterization of gold in the cytosol of rat-kidney cortex cells

✍ Scribed by Howard O. Thompson; Judith Blaszak; Connie J. Knudtson; C.Frank Shaw III

• Book ID: 104105929
• Publisher: Elsevier Science
• Year: 1978
• Weight: 846 KB
• Volume: 9
• Category: Article
• ISSN: 0006-3061
• DOI: 10.1016/s0006-3061(00)80147-7

✦ Synopsis

Evidence is presented for a chemical equilibrium of gold(I) among suKhydryl binding sites in the cytoplasm of renal cortical celIs of the rat. The goId dktriiution among protein and nonprotein species, examined by gel-penneation chromatography following in Vito administration of gold sodium thio-maIate, gold sodium thioghxcose, NaAuCh, and QH&PAuCl, was similar in each case. although the cytoplasmic gold concentrations varied The pattern of gold elution closely parallels that of the thiol groups in the supematant. In vitro addition of gold sodium thiomalate to the supematant fraction produced an identical distriiution. Addition of cysteine to cytoplasm foRowing in a&o administration of gold drastically decreased the protein-bound gold and increased the non-protein-bound gold. These data rule out the induced synthesis of a metallothioneinae gold-binding protein. Ultracentrihgation revealed goId to be present in the microsomal fraction as well as the supematant.