Characterization of glucose oxidase-negative mutants of a lignin degrading basidiomycetePhanerochaete chrysosporium

✍ Scribed by R. L. Kelley; K. Ramasamy; C. Adinarayana Reddy

• Publisher: Springer
• Year: 1986
• Tongue: English
• Weight: 471 KB
• Volume: 144
• Category: Article
• ISSN: 0302-8933
• DOI: 10.1007/bf00410958

✦ Synopsis

The isolation and characterization of glucose oxidase-negative (gox-) mutants of Phanerochaete chrysosporium, is described. These mutants are deficient not only in their ability to produce hydrogen peroxide (H202) but also in lignin degradation (2'-14C-synthetic lignin ~ 14CO2), Iigninase and peroxidase activities, decolorization of the dye poly-R 481, and production of ethylene from e-oxo-7methylthiobutyric acid (KTBA). The gox-mutants retained, albeit at a lower level, the capacity to produce veratryl alcohol, a typical secondary metabolite, and produced conidia at a level comparable to that of the wild type. The addition of ligninase and/or glucose oxidase to a goxmutant (GOX-10) did not enhance its capacity to degrade lignin. The Gox + revertant strains regained glucose oxidase activity, the ability to degrade lignin, as well as the other characteristics that were missing in the gox-mutants. The results suggest that the genetic lesion in these mutants affects the regulation of a set of secondary metabolic characteristics.