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Characterization of galactooligosaccharides produced by β-galactosidase immobilized onto magnetized Dacron

✍ Scribed by David F.M. Neri; Victor M. Balcão; Susana M. Cardoso; Artur M.S. Silva; Maria do Rosário M. Domingues; Duarte P.M. Torres; Lígia R.M. Rodrigues; Luiz B. Carvalho Jr.; José A.C. Teixeira


Book ID
116540588
Publisher
Elsevier Science
Year
2011
Tongue
English
Weight
719 KB
Volume
21
Category
Article
ISSN
0958-6946

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## Abstract β‐Galactosidase was immobilized on chitosan using tris(hydroxymethyl)phosphine (THP) as a coupling agent to produce galactooligosaccharides (GOS) from lactose. Both the THP‐immobilized and the free enzymes were maximally achieved at pH 5.0 and the optimal temperature was 55 °C. The resi