Characterization of G3BPs: Tissue specific expression, chromosomal localisation and rasGAP120 binding studies

✍ Scribed by Derek Kennedy; Juliet French; Estelle Guitard; Kelin Ru; Bruno Tocque; John Mattick

• Book ID: 102875763
• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 672 KB
• Volume: 84
• Category: Article
• ISSN: 0730-2312
• DOI: 10.1002/jcb.1277

✦ Synopsis

Abstract

The G3BP (ras‐GTPase‐Activating Protein SH3‐Domain‐Binding Protein) family of proteins has been implicated in both signal transduction and RNA‐metabolism. We have previously identified human G3BP‐1, G3BP‐2, and mouse G3BP‐2. Here, we report the cloning of mouse G3BP‐1, the discovery of two alternatively spliced isoforms of mouse, and human G3BP‐2 (G3BP‐2a and G3BP‐2b), and the chromosomal localisation of human G3BP‐1 and G3BP‐2, which map to 5q14.2‐5q33.3 and 4q12‐4q24 respectively. We mapped the __ras__GAP^120^ interactive region of the G3BP‐2 isoforms and show that both G3BP‐2a and G3BP‐2b use an N‐terminal NTF2‐like domain for __ras__GAP^120^ binding rather than several available proline‐rich (PxxP) motifs found in members of the G3BPs. Furthermore, we have characterized the protein expression of both G3BP‐1 and G3BP‐2a/b in adult mouse tissues, and show them to be both tissue and isoform specific. J. Cell. Biochem. 84: 173–187, 2002. © 2001 Wiley‐Liss, Inc.