Characterization of fall armyworm mitochondrial DNA (Lepidoptera: Noctuidae)

✍ Scribed by Li Dao Ke; Dorothy P. Pashley

• Book ID: 101603889
• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 309 KB
• Volume: 21
• Category: Article
• ISSN: 0739-4462
• DOI: 10.1002/arch.940210403

✦ Synopsis

Abstract

Mitochondrial DNA from the fall armyworm, Spodoptera frugiperda (J.E. Smith), was cloned and characterized using restriction enzyme mapping. Genome size is approximately 16.3 kilobase (Kb), consistent with that of most animals. Three fragments, 8.1 Kb, 6.2 Kb, and 2.0 Kb, were produced by digestion with restriction enzyme __Xba__l and cloned into a pUC19 vector. Twenty‐nine restriction enzymes were used to generate a detailed physical restriction enzyme map of the three cloned fragments. These data represent the first detailed characterization of a lepidopteran mitochondrial genome. © 1992 Wiley‐Liss, Inc.