Characterization of ethanol-extractable reduced subunits of glutenin separated by reversed-phase high-performance liquid chromatography
โ Scribed by Wieser, H.; Seilmeier, W.; Belitz, H.-D.
- Book ID
- 123188512
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 663 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0733-5210
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The subunits of glutenin, the disulfide-bonded, high-molecular weight protein fraction of wheat, have been separated by reversed-phase high-performance liquid chromatography (RP-HPLC) on large-pore silica-based columns. Glutenin was first isolated from bulk samples, and effects of dissociating, redu
Baseline separation of subunits of diverse phycobiliproteins was achieved by a reverse-phase HPLC gradient method with a C4 large-pore column and a solvent system consisting of 0.1% trifluoroacetic acid (TFA) in water and 0.1% TFA in 2:1 (v/v) acetonitrile:isopropanol. The procedure was successfully