The E. coli recA gene was cloned from the phage lambda precA into the vector pBR313. A plasmid, pJL3, was also isolated by cloning a portion of the recA gene into the vector pBR322. pJL3 coded for a fragment of the recA protein 34 Kd (kilodaltons) in size (compared to 40 Kd for the intact protein).
Characterization of Escherichia coli plasmid coding for hydrogen sulfide production
✍ Scribed by Józef Mleczko; Marian Mulczyk
- Book ID
- 109312027
- Publisher
- John Wiley and Sons
- Year
- 1983
- Tongue
- English
- Weight
- 275 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0378-1097
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
Tetracycline resistance and hydrogen sulfide production have been previously found to be plasmid-mediated in a naturally-occurring strain of Escherichia coli; both functions are specified by a single conjugative plasmid called pIP231(Te-H2S); pIP231DNA was isolated as covalently closed molecules in
The plasmid pIP231, determining tetracycline resistance and hydrogen sulfide production is shown to belong to incompatibility group Y and to code for a restriction and modification system. Unlike the IncY plasmids, P7 and P15B, plasmid pIP231 shows only little genetic and physical homology with P1 p