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Characterization of Escherichia coli plasmid coding for hydrogen sulfide production

✍ Scribed by Józef Mleczko; Marian Mulczyk


Book ID
109312027
Publisher
John Wiley and Sons
Year
1983
Tongue
English
Weight
275 KB
Volume
20
Category
Article
ISSN
0378-1097

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The E. coli recA gene was cloned from the phage lambda precA into the vector pBR313. A plasmid, pJL3, was also isolated by cloning a portion of the recA gene into the vector pBR322. pJL3 coded for a fragment of the recA protein 34 Kd (kilodaltons) in size (compared to 40 Kd for the intact protein).

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The plasmid pIP231, determining tetracycline resistance and hydrogen sulfide production is shown to belong to incompatibility group Y and to code for a restriction and modification system. Unlike the IncY plasmids, P7 and P15B, plasmid pIP231 shows only little genetic and physical homology with P1 p