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Characterization of erythropoietin-like activity from mouse spleen cells

✍ Scribed by Barbara Fagg; Carolyn A. Roitsch

Publisher
John Wiley and Sons
Year
1986
Tongue
English
Weight
799 KB
Volume
126
Category
Article
ISSN
0021-9541

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✦ Synopsis

Jlausc Kp and spleen cell conditioned medium Anemic mouse serum was prepared as described by Tamhourin et al. 11973). Mice were X-irradiated (600 Radsi. injected intraperitoneally with 60 mgkg of phenylhydrazine on the following day, and bled on the tenth day, by which time their hematocrit had decreased to 10-25'2. The serum, which contained 10-30 U Ep/ml, was filter-sterilized and stored at -20Β°C.

Spleen cell conditioned medium was harvested from secondary mixed lymphocyte cultures (Ryser et al., 1978).

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