Characterization of enzymes involved in biotransformation of polycyclic aromatic hydrocarbons in terrestrial isopods
β Scribed by Joop A. de Knecht; Gerard J. Stroomberg; Cornelis Tump; Martine Helms; Rudo A. Verweij; Jan Commandeur; Cornelis A. M. van Gestel; Nico M. van Straalen
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 88 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0730-7268
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## Abstract In this study, biotransformation products of pyrene were measured in the hepatopancreas of terrestrial isopods as biomarkers of polycyclic aromatic hydrocarbon (PAH) exposure. These productsβpyreneβ1βglucoside, pyreneβ1βsulfate, an unknown pyrene conjugate, and 1βhydroxypyreneβwere quan
The fluorescence intensity of polycyclic aromatic hydrocarbons (PAHs) is higher in Brij-35 micellar solution than in methanol. Pyrene, benzc$ghilperylene and benzo[a]pyrene could be determined in the presence of up to a lOO-fold excess of other PAHs, the errors being lower than 5%. The relative stan