Characterization of enzymatic D-xylulose 5-phosphate synthesis

✍ Scribed by Jobin Shaeri; Ian Wright; Elner B. Rathbone; Roland Wohlgemuth; John M. Woodley

• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 284 KB
• Volume: 101
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.21949

✦ Synopsis

Abstract

In this article we report on the characterization of the enzymatic synthesis of D‐xylulose 5‐phosphate using triosephosphate isomerase and transketolase. Two potential starting substrates are possible with this scheme. The data presented here allow a comparison of both routes for the synthesis, based on experimental information on reaction kinetics. Operational guidelines are proposed which should assist in the scale‐up of such syntheses. Biotechnol. Bioeng. 2008;101: 761–767. © 2008 Wiley Periodicals, Inc.