Characterization of ebv-carrying b-cell populations in healthy seropositive individuals with regard to density, release of transforming virus and spontaneous outgrowth
✍ Scribed by Nongnit Lewin; Pierre Åman; Maria Grazia Masucci; Eva Klein; George Klein; Bo Öberg; Hans Strander; Werner Henle; Gertrude Henle
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- French
- Weight
- 531 KB
- Volume
- 39
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Peripheral or tonsil lymphocyte populations of EBV-sero-have not been characterized. They may or may not be identipositive donors give rise to EBv-CaryYing LcLS upon in VitrO cal. Our study is an attempt towards their definition. We have explantation. Such lines can either by a 2-step mecha-separated resting and activated B-cell populations from healthy nismp namely release Of virus from SOme Of the EBV-seropositive donors on density gradients and explanted cells followed by infection of previously uninfected B cells, or them in the presence of cord-blood cells of the opposite sex. by direct outgrowth of virus-harboring B cells (Rickinson et a/., 1974; Dalens eta/., 1975; Hinuma and Katsuki 1978; Katsuki Cyclosporin A (CSA) was added, in Order to the funceta/., 1979). We observed that cells responsible for both the 2-tion of the remaining T cells (Bird et al., 1981; Rickinson et step mechanism and for direct outgrowth are found in the al., 1984). The donor VS. cord-blood origin of the LCLs was purified B-cell compartment. Virus release was more frequent determined by sex chromosome typing. The anti-viral agent, than direct outgrowth. The majority of virus-releasing cells phosphonoformate (PFA) and/or virus-neutralizing human anwere found in the low-density fraction that contains large, tibodies were added in Some of our experiments in order to activated blasts-that were
Of spontaneous prevent virus release and subsequent transformation of the outgrowth in the presence of the viral inhibitor PFA and of cord-blo& lymphocytes.
virus-neutralizing antibody gave rise to cell lines that carried the sex chromosome marker of the original donor, rather than that of admixed cord blood lymphocyte of the opposite sex. Such cells were found in both the low-and the highdensity fractions. The majority of the EBV-carrying B cells in vivo are thus low-density blasts. Rare small B cells of high density harboring EBV were capable Of spontaneous outgrowth. This may be indicative of a host control mechanism that is removed upon cultivation in vitro.