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Characterization of DNA-Binding Proteins Using Multiplexed Competitor EMSA

โœ Scribed by Andrew J.P. Smith; Steve E. Humphries

Book ID
116663954
Publisher
Elsevier Science
Year
2009
Tongue
English
Weight
191 KB
Volume
385
Category
Article
ISSN
0022-2836

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When a polypeptide binds to DNA, the rotational mobility of a chromophore on the polypeptide is reduced. This simple fact makes it feasible to use polarization of fluorescence methods to study polypeptide-nucleic acid interaction. Previous (l-3) polarization of fluorescence studies of polypeptide-DN