✦ LIBER ✦

Characterization of cymbidium mosaic virus coat protein gene and its expression in transgenic tobacco plants

✍ Scribed by Tet-Fatt Chia; Yang-Sun Chan; Nam-Hai Chua

Publisher: Springer
Year: 1992
Tongue: English
Weight: 883 KB
Volume: 18
Category: Article
ISSN: 0167-4412
DOI: 10.1007/bf00047712

No coin nor oath required. For personal study only.

✦ Synopsis

Cymbidium mosaic virus (CyMV) is the most prevalent virus infecting orchids. Here, we report the isolation of partial cDNA clones encoding the genomic RNA of CyMV. Like most of the polyadenylated monopartite positive-strand RNA viruses, the open reading frame (ORF) coding for the viral coat protein (CP) is located at the 3' end. The ORF predicts a polypeptide chain of 220 amino acids with a molecular weight of 23,600. Sequence comparison of this ORF to the CP sequences of potato virus X(PVX) and white clover mosaic virus (WClMV) revealed a strong amino acid homology in the mid-portion of the CP, but the overall homology was low. The CyMV CP gene was placed downstream of a cauliflower mosaic virus 35S promoter and the chimaeric gene was transferred into Nicotiana benthamiana. Transgenic plants expressing the CyMV CP were protected against CyMV infection.

📜 SIMILAR VOLUMES

Relationship between transcript producti

Relationship between transcript production and virus resistance in transgenic tobacco expressing the potato leafroll virus coat protein gene

✍ H. Barker; B. Reavy; K. D. Webster; C. A. Jolly; A. Kumar; M. A. Mayo 📂 Article 📅 1993 🏛 Springer 🌐 English ⚖ 686 KB

The coat protein (CP) gene of potato leafroll luteovims (PLRV) was inserted into tobacco (Nicotiana tabacum) using disarmed Agrobacterium tumefaciens (LBA4404) containing a binary expression vector. PLRV CP gene transcript was detected in transgenic plants but its abundance differed between transfor

Cauliflower mosaic virus gene VI causes

Cauliflower mosaic virus gene VI causes growth suppression, development of necrotic spots and expression of defence-related genes in transgenic tobacco plants

✍ Takahashi, Hideki ;Shimamoto, Ko ;Ehara, Yoshio 📂 Article 📅 1989 🏛 Springer 🌐 English ⚖ 762 KB

Constitutive transcription of a soybean

Constitutive transcription of a soybean heat-shock gene by a cauliflower mosaic virus promoter in transgenic tobacco plants

✍ Schöffl, Fritz ;Rieping, Mechthild ;Baumann, Götz 📂 Article 📅 1987 🏛 John Wiley and Sons 🌐 English ⚖ 645 KB

Characterization of two class II chitina

Characterization of two class II chitinase genes from peanut and expression studies in transgenic tobacco plants

✍ Jan-Wolfhard Kellmann; Tatjana Kleinow; Kerstin Engelhardt; Christina Philipp; D 📂 Article 📅 1996 🏛 Springer 🌐 English ⚖ 847 KB

Two different genes encoding class II chitinases from peanut (Arachis hypogaea L. cv. NC4), A.h.Chi2;l and A.h.Chi2;2, have been cloned. In peanut cell suspension cultures, mRNA levels of A.h.Chi2;2 increased after ethylene or salicylate treatment and in the presence of conidia from Botrytis cinerea

Characterization of the catalase antioxi

Characterization of the catalase antioxidant defense gene Cat1 of maize, and its developmentally regulated expression in transgenic tobacco

✍ Lingqiang Guan; John G. Scandalios 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 English ⚖ 919 KB

A genomic clone containing the maize __Cat1__ gene has been isolated and its complete DNA sequence determined. The start of transcription has been mapped by primer extension. Six introns were identified in the __Cat1__ coding region. In order to determine the tissue‐specific expression pattern of th

Isolation of a ubiquitin-ribosomal prote

Isolation of a ubiquitin-ribosomal protein gene (ubi3) from potato and expression of its promoter in transgenic plants

✍ Joan E. Garbarino; William R. Belknap 📂 Article 📅 1994 🏛 Springer 🌐 English ⚖ 878 KB

A genomic clone encoding the potato homolog of the yeast ubiquitin-ribosomal protein fusion gene ubi3 was isolated and characterized. Chimeric genes containing the ubi3 promoter (920 bp of 5' to the ubiquitin start codon) were constructed in which the reporter gene/~-glucuronidase (GUS) was either f