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Characterization of class I bovine lymphocyte antigens (BoLA) by one-dimensional isoelectricfocusing

✍ Scribed by I. JOOSTEN; R. A. OLIVER; R. L. SPOONER; J. L. WILLIAMS; B. G. HEPKEMA; M. F. SANDERS; E. J. HENSEN


Book ID
114705505
Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
646 KB
Volume
19
Category
Article
ISSN
0268-9146

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A procedure for the molecular identification of MHC class I products based on 1-D IEF and subsequent immunoblotting is described. Optimal conditions for 1-D IEF, the electrophoretic transfer of proteins out of denaturing, nonionic detergent-containing gels to nitrocellulose, and the requisite antibo