Characterization of cellular attachment and spreading molecules at liquid-liquid interfaces

✍ Scribed by Steven Sobel; Murray D. Rosenberg

• Publisher: Elsevier Science
• Year: 1984
• Tongue: English
• Weight: 715 KB
• Volume: 140
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(84)90198-2

✦ Synopsis

Cells are grown at a liquid-liquid interface (ultrafiltrate of growth medium--heptacosafluorotributylamine). Following attachment and spreading an intermediate-density, isotonic solution of metrizamide-Ficoll is introduced. Cells are detached by centrifugation at 150,000g X 45 min. Molecules deposited at the interface are removed and examined by gel electrophoresis. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis reveals 20 protein bands ranging in molecular weight from 30,000 to 200,000. Most bands have molecular weights less than 120,000. The substrate-attached material contains approximately 2.8 pg of protein per attached cell. Substrate-attached molecules can be removed without detergent denaturation for additional studies.