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Characterization of C4-2 as a tumor-suppressor gene in human brain tumors

✍ Scribed by Sehgal, Anil; Keener, Cassie; Boynton, Alton L.; Young, Ronald F.; Vermeulen, Sandra S.; Yonemura, Kenneth S.; Kohler, Erik P.; Aldape, Hector C.; Simrell, Charles R.; Murphy, Gerald P.

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 189 KB
Volume: 64
Category: Article
ISSN: 0022-4790
DOI: 10.1002/(sici)1096-9098(199702)64:2<102::aid-jso3>3.0.co;2-f

No coin nor oath required. For personal study only.

✦ Synopsis

Background: Brain tumors claimed the lives of 13,300 people in 1995. Our objective was to isolate and characterize unique tumor-suppressor genes from human brain tumors derived from patients in the United States. Methods: Differential display-polymerase chain reaction was used to isolate tumor suppressor genes. Results: Clone C4-2 was isolated and is expressed in normal adult human brain, but not in brain tissue from glioblastoma multiforme tumors. C4-2 has 66% homology to the previously isolated ARPP-16 (cAMP-regulated phosphoprotein of Mr ‫ס‬ 16,000) based on limited sequencing. C4-2 is expressed at high levels in normal brain and is not expressed or expressed at low levels in several brain tumor cell lines. Expression of C4-2 was also either not expressed or expressed at low levels in meningioma, B-cell lymphoma, recurrent glioma, LNCAP (prostate tumor cell line), breast tumor, or prostate tumor tissue.

Conclusion:

We conclude that C4-2 may function as a potential tumorsuppressor gene.

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