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Characterization of Brightness and Stoichiometry of Bright Particles by Flow-Fluorescence Fluctuation Spectroscopy

✍ Scribed by Johnson, Jolene; Chen, Yan; Mueller, Joachim D.

Book ID
119207537
Publisher
Biophysical Society
Year
2010
Tongue
English
Weight
534 KB
Volume
99
Category
Article
ISSN
0006-3495

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## Abstract Experiments based on fluorescence detection are limited by the population of the fluorescence marker’s long‐lived dark triplet state, leading to pronounced photobleaching reactions and blinking which reduces the average fluorescence signal obtained per time interval. By irradiation with