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Characterization of azadirachtin binding to Sf9 nuclei in vitro

โœ Scribed by Alasdair J. Nisbet; A. Jennifer Mordue (Luntz); Robert B. Grossman; Lyn Jennens; Steven V. Ley; William Mordue


Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
126 KB
Volume
46
Category
Article
ISSN
0739-4462

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Characterization of azadirachtin binding
โœ Alasdair J. Nisbet; A. Jennifer Mordue (Luntz); Robert B. Grossman; Lyn Jennens; ๐Ÿ“‚ Article ๐Ÿ“… 1997 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 91 KB

22,23-3 H 2 ]dihydroazadirachtin was incorporated by Sf9 cells in culture and was bound specifically to the nuclear fraction. The observed association constant of the binding of the radioligand to a purified nuclear fraction was determined to be 0.037 ยฑ 0.008 min -1 using a one-phase exponential ass

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## Abstract Heat precipitation fibril formation in collagen solutions depends upon the prior thermal history of the solution. Collagen solutions were heat precipitated to various extents at 30ยฐC, cooled, and then brought to a second precipitation. Kinetic analysis of the secondary precipitation dem