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Characterization of antibody aggregation: Role of buried, unpaired cysteines in particle formation

โœ Scribed by Brych, Stephen R. (author);Gokarn, Yatin R. (author);Hultgen, Heather (author);Stevenson, Riki J. (author);Rajan, Rahul (author);Matsumura, Masazumi (author)


Publisher
John Wiley and Sons Inc.
Year
2010
Tongue
English
Weight
479 KB
Volume
99
Category
Article
ISSN
0022-3549

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Characterization of antibody aggregation
โœ Brych, Stephen R. (author);Gokarn, Yatin R. (author);Hultgen, Heather (author);S ๐Ÿ“‚ Article ๐Ÿ“… 2010 ๐Ÿ› John Wiley and Sons Inc. ๐ŸŒ English โš– 479 KB

Proteins are susceptible to degradation upon exposure to a variety of stresses during product manufacturing, transportation and storage. In this study, we investigated the aggregation properties of a monoclonal antibody during agitation stress. Agitation exclusively led to insoluble aggregates, or p