✦ LIBER ✦

Characterization of anti-N-acetyl-d-glucosamine antibodies elicited through haptenated liposomes

✍ Scribed by Manoj K. Das; Samir K. Roy; Debi Prasad Sarkar

Publisher: Elsevier Science
Year: 1984
Tongue: English
Weight: 470 KB
Volume: 128
Category: Article
ISSN: 0008-6215
DOI: 10.1016/0008-6215(84)85340-9

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✦ Synopsis

Antiserum was raised in rabbits against p-aminophenyl 2-acetamido-2-deoxy-beta-D-glucoside covalently coupled to phosphatidylaminoethanol-containing lecithin liposomes. The affinity-purified antibodies were found to be IgM. The properties of the antibodies were studied by ligand inhibition of quantitative precipitation, fluorescence titration with 2-acetamido-2-deoxy-D-glucose, and complement lysis of haptenated liposomes.