Characterization of an inward-rectifying potassium current in NG108-15 neuroblastoma×glioma cells
✍ Scribed by Qian Hu; Y. L. Shi
- Book ID
- 106111209
- Publisher
- Springer
- Year
- 1997
- Tongue
- English
- Weight
- 710 KB
- Volume
- 433
- Category
- Article
- ISSN
- 0031-6768
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The M current, IM, a voltage-dependent noninactivating K current, was recorded in NG108-15 neuroblastoma x glioma hybrid cells, using the whole-cell mode of the patch-clamp technique. We studied inhibition of the M current by bradykinin, phorbol dibutyrate (PDBu), an activator of protein kinase C (P
The whole-cell patch-clamp technique was used to assess the current carried by inwardly rectifying K ؉ channels (K ir ) and the resting membrane potential (RMP) during long-term culture of NG108-15 cells. Culture of this cell line in serum-free medium triggers differentiation of a type I, neuron-lik