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Characterization of an Extremely Thermophilic and Oxygen-Stable Membrane-Bound Hydrogenase from a Marine Hydrogen-Oxidizing BacteriumHydrogenovibrio marinus

โœ Scribed by Hirofumi Nishihara; Youji Miyashita; Katsuhiro Aoyama; Tohru Kodama; Yasuo Igarashi; Yoshichika Takamura


Book ID
115580031
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
116 KB
Volume
232
Category
Article
ISSN
0006-291X

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Purification and characterization of a h
โœ Ki-Seok Yoon; Keiichi Fukuda; Kiyoshi Fujisawa; Hirofumi Nishihara ๐Ÿ“‚ Article ๐Ÿ“… 2011 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 391 KB

The membrane-bound [NiFe]-hydrogenase from Hydrogenovibrio marinus (HmMBH) was purified homogeneously under anaerobic conditions. Its molecular weight was estimated as 110 kDa, consisting of a heterodimeric structure of 66 kDa and 37 kDa subunits. The purified enzyme exhibited high activity in a wid