Characterization of an extract from the leaves of Cissampelos sympodialis Eichl. on the spontaneous tone of isolated trachea

✍ Scribed by George Thomas; Fiona Burns; Susan Pyne; Nigel J. Pyne

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 57 KB
• Volume: 11
• Category: Article
• ISSN: 0951-418X
• DOI: 10.1002/(sici)1099-1573(199711)11:7<496::aid-ptr137>3.0.co;2-7

✦ Synopsis

An aqueous fraction of the ethanol extract of the leaves (AFL) of Cissampelos sympodialis Eichl. (Menispermaceae) was shown to relax the spontaneous tone of guinea-pig tracheal smooth muscle rings with an IC 50 of 20.5 g/mL. The AFL inhibited cyclic nucleotide phosphodiesterase activity in isolated smooth muscle homogenates and stimulated an increase in intracellular cAMP synthesis in intact cultured smooth muscle cells. A complete inhibition of phosphodiesterase activity in isolated homogenates was almost achieved. These studies indicate that the AFL contains a substance which is a non-selective, but highly potent inhibitor of phosphodiesterase activity. This probably accounts for its effect on the spontaneous tone of guinea-pig tracheal smooth muscle.