Characterization of an early cytotoxicity-inducing factor in sera of japanese quails after inoculation with rous sarcoma virus
✍ Scribed by Yasuhiro Yoshikawa; Kazuya Yamanouchi; Akiko Fukuda; Masanori Hayami
- Publisher
- John Wiley and Sons
- Year
- 1976
- Tongue
- French
- Weight
- 641 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Abstract
By pre‐treatment with serum of normal spleen cells used in the microcytotoxicity assay, a humoral factor which induces cytotoxic activity in normal spleen cells was demonstrated in about 40% of sera of Japanese quails as early as 3 days after inoculation with Rous sarcoma virus (RSV). This cytotoxicity‐inducing activity was not present either in the IgM or the IgG fraction obtained by Sephadex gel filtration. In sera of quails with experimentally induced agammaglobulinemia, the cytotoxicity‐inducing activity was demonstrated at the same frequency as in normal animals. Thus, it seems unlikely that the early cytotoxicity‐inducing factor is an immunoglobulin. On the other hand, membrane fractions extracted with 3 m potassium chloride from the RSV‐induced quail tumor (QT) cells used as target cells in the microcytotoxicity assay exhibited cytotoxicity‐inducing activity. After spontaneous regression of an RSV‐induced tumor the serum of the regressor quail contained antibodies specific to the QT cell extract. This serum removed the cytotoxicity‐inducing activity of both QT cell extract and serum sampled 3 days after RSV inoculation. In contrast, serum without antibody to the QT cell extract failed to absorb the cytotoxicity‐inducing activity of the extract. It is therefore suggested that soluble tumor antigens can act as an early cytotoxicity‐inducing factor.