Characterization of ADP-glucose pyrophosphorylase fromshrunken-2andbrittle-2mutants of maize

✍ Scribed by L. C. Hannah; O. E. Nelson

• Book ID: 104784945
• Publisher: Springer
• Year: 1976
• Tongue: English
• Weight: 694 KB
• Volume: 14
• Category: Article
• ISSN: 0006-2928
• DOI: 10.1007/bf00485834

✦ Synopsis

Electrophoretic characterization of adenosine diphosphate glucose pyrophosphorylase from the developing endosperms of nine shrunken-2 and four brittle-2 mutants revealed that (1) all mutants had low but detectable levels of activity, (2) mutation at either locus decreased activity of pyrophosphorylases A and B, and (3) differences in mobility were not found. However, pyrophosphorylase B extracted from several shrunken-2 and brittle-2 mutants differed from normal in extent of urea denaturation, Km (glucose-1-phosphate) or type of glucose-1-phosphaociation with the sh2 locus) appears to differ from normal in Km (glucose-1-phosphate).