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Characterization of activatable form of prolyl hydroxylase in L929 fibroblasts

โœ Scribed by Ramadasan Kuttan


Book ID
113124332
Publisher
Elsevier Science
Year
1981
Weight
601 KB
Volume
660
Category
Article
ISSN
0005-2744

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Assay of prolyl hydroxylase in cultured
โœ Joel S. Trupin; James D. Russell; Shirley B. Russell ๐Ÿ“‚ Article ๐Ÿ“… 1982 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 364 KB

Prolyl hydroxylase may be assayed in cultured cell monolayers after a cold acetone treatment or air-drying. Enzyme activity of dried cells is stable when cell monolayers are stored frozen and desiccated, thus permitting simultaneous assay of cells harvested at different times, e.g., on different day