Characterization of acid phosphatase-1 null activity mutants ofDrosophila melanogaster

✍ Scribed by John Bell; Ross MacIntyre

• Publisher: Springer
• Year: 1973
• Tongue: English
• Weight: 965 KB
• Volume: 10
• Category: Article
• ISSN: 0006-2928
• DOI: 10.1007/bf00485747

✦ Synopsis

A complementation analysis wasperformed on 15 Acph-1 n alleles. Only three of these alleles pro red to be nonleaky and to exhibit no evidence of eomplementation. These were then tested immunologically to determine their level of antigenically cross-reacting material (CRM). Their CRM levels were virtually zero, as compared to low~ but positive, levels for two other homozygous Acph-1" mutants and considerably higher levels for heteroallelic combinations of some of the leaky alleles. Acid phosphatase-1 enzyme subunits, formed by dissociating native enzyme, have close to 100% CRM activity in tests with antibodies eBcited by native enzyme.