Characterization of a yeast replication origin (ars2) and construction of stable minichromosomes containing cloned yeast centromere DNA (CEN3)

A hybrid DNA sequence containing the rep

A hybrid DNA sequence containing the replication origin of the multicopy yeast plasmid 2 μm circle and an additional repeated sequence can convert maltose-negative into maltose-positive strains

✍ Rodicio, R. ;Schmitt, H. -D. ;Heinisch, J. ;Zimmermann, F. K. 📂 Article 📅 1984 🏛 Springer 🌐 English ⚖ 761 KB

Yeast DNA pools were prepared by ligating partial Sau3A genomic digests from strains carrying various MAL genes into the BamHI site of the yeast-Escherichia coli shuttle vector YRp7. They were used to transform recipient yeast strains that could not utilize maltose since they lacked a classical MAL