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Characterization of a type-C virus produced by co-cultures of human leukemic bone-marrow and fetal canine thymus cells

✍ Scribed by R. Guy Smith; Kees Nooter; Peter Bentvelzen; M. Robert-Guroff; K. Harewood; M. S. Reitz; Sin Ai Lee; R. C. Gallo


Publisher
John Wiley and Sons
Year
1979
Tongue
French
Weight
752 KB
Volume
24
Category
Article
ISSN
0020-7136

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✦ Synopsis


Abstract

The putative human helper virus SKA‐21/A204V, isolated by Nooter et aal. in 1977 from human leukemic bone‐marrow cells following co‐culture with normal fetal canine thymus cells, Cf2th, has been characterized with respect to its major viral core protein, reverse transcriptase, and nucleic acid sequences. The results of these analyses show that this virus is not distinguishable from the woolly monkey type‐C virus, SSAV‐1, by the techniques employed.


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Detection of human c-type “helper” virus
✍ Kees Nooter; Peter Bentvelzen; Chris Zurcher; Johng Rhim 📂 Article 📅 1977 🏛 John Wiley and Sons 🌐 French ⚖ 694 KB

## Abstract Bone‐marrow cells from two leukemic children were co‐cultivated with the canine thymus cell line A 7573. In early passages, C‐type oncornaviruses were released as detected by extracellular reverse transcriptase assay. Co‐cultivation of the infected canine cells with the non‐producing ce