Characterization of a transmissible growth-promoting agent derived from CSF of schizophrenic patients which is active on human neuroblastoma cells
✍ Scribed by Dr. Susumu Shirabe; J. R. Stevens; J. P. Schwartz
- Book ID
- 102908530
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 945 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0360-4012
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✦ Synopsis
A growth-promoting agent for the human neuroblastoma cell line SK-N-SH(EP) (SH-EP) has been detected in human cerebrospinal fluid (CSF) derived from schizophrenic patients. Following treatment with the CSF, a number of properties of the SH-EP cells changed permanently. These included an accelerated rate of growth, an increased cell density at confluence, a change of cell shape, and an increased ability to form colonies in soft agar. All of these changes are consistent with further cellular transformation of the SH-EP cells. Once the cells' properties had changed following CSF treatment, the growthpromoting activity was found to be present in freezethawed cell extracts and in the culture medium, and could be passed to untreated SH-EP cells. The activity could be detected in culture media diluted as high as lo8. It was inactivated by proteinases, chloroform, or heat but passed through a 0.22-pm filter. The growth-promoting activity can be banded on a Percoll gradient, suggesting that it is particulate rather than a soluble growth factor.