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Characterization of a thiamin diphosphate-dependent phenylpyruvate decarboxylase from Saccharomyces cerevisiae

✍ Scribed by Malea M. Kneen; Razvan Stan; Alejandra Yep; Ryan P. Tyler; Choedchai Saehuan; Michael J. McLeish

Book ID: 111313907
Publisher: John Wiley and Sons
Year: 2011
Tongue: English
Weight: 276 KB
Volume: 278
Category: Article
ISSN: 1432-1327
DOI: 10.1111/j.1742-4658.2011.08103.x

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## Abstract The thiamine diphosphate‐dependent, branched‐chain 2‐keto acid decarboxylase from __Lactococcus lactis__ sup. __cremoris__ B1157 (KdcA) is a new valuable enzyme for the synthesis of chiral 2‐hydroxy ketones. The gene was cloned and the enzyme was expressed as an N‐terminal hexahistidine