The benzoyl-CoA ligase from an anaerobic syntrophic culture was purified to homogeneity. It had a molecular mass of around 420 kDa and consisted of seven or eight subunits of 58 kDa. The temperature optim u m was 37-40 Β° C, the optimum pH around 8.0 and optimal activity required 50-100 mM TRIS-HC1 b
Characterization of a succinate-fermenting anaerobic bacterium isolated from a glycolate-degrading mixed culture
β Scribed by Peter H. Janssen
- Publisher
- Springer
- Year
- 1991
- Tongue
- English
- Weight
- 660 KB
- Volume
- 155
- Category
- Article
- ISSN
- 0302-8933
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## Abstract A 2βhaloacid dehalogenaseβproducing bacterium, designated DEH99, was isolated from the marine sponge __Hymeniacidon perlevis__ using a modified enrichment medium and a pH indicator method. DEH99 could degrade only half of the racemic mixture 2βchloropropionic acid (2βCPA) in the medium.
## Abstract The study investigated the effects of adding an anaerobic fungus (__Piromyces__ sp FNG5; isolated from the faeces of a wild blue bull) to the rumen fluid of buffaloes consuming a basal diet of wheat straw and concentrates on __in vitro__ enzyme activities, fermentation and degradation o