Characterization of a spontaneously arising murine squamous cell carcinoma (SCC VII) as a prerequisite for head and neck cancer immunotherapy

✍ Scribed by Dianne Khurana; Elizabeth A. Martin; Jan L. Kasperbauer; Bert W. O'Malley Jr; Diva R. Salomao; Lieping Chen; Scott E. Strome

• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 237 KB
• Volume: 23
• Category: Article
• ISSN: 1043-3074
• DOI: 10.1002/hed.1130

✦ Synopsis

Abstract

Background

To develop novel therapeutic approaches for patients with head and neck malignancies, poorly immunogenic murine models of squamous cell carcinoma (SCC) need to be defined.

Methods

The phenotype, growth characteristics, and responsiveness to tumor‐specific T‐cell transfer of a spontaneously arising murine SCC (SCC VII) were characterized.

Results

SCC VII expresses major histocompatibility complex (MHC) class I molecules yet is resistant to tumor‐specific T‐cell killing and relatively insensitive to killing mediated by lymphokine‐activated killer (LAK) cells. Intradermal tumors are reproducibly established after vaccination of 5 × 10^4^ cells, and systemic micrometastases are apparent after intravenous administration of 2.5 × 10^4^ cells. Immunotherapy of 3‐day lung metastases using tumor‐specific T cells and systemic interleukin‐2 (IL‐2) was ineffective in reducing the number of metastases in vivo.

Conclusions

SCC VII is a poorly immunogenic murine squamous cell cancer, which represents an ideal model for preclinical testing of immunotherapeutic approaches for patients with SCC of the upper aerodigestive tract. © 2001 John Wiley & Sons, Inc. Head Neck 23: 899–906, 2001.