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Characterization of a spontaneous mutant of Azotobacter vinelandii in which vanadium-dependent nitrogen fixation is not inhibited by molybdenum

✍ Scribed by Umesh K Bageshwar; Ramesh Raina; H.K Das

Book ID: 117518618
Publisher: John Wiley and Sons
Year: 1998
Tongue: English
Weight: 158 KB
Volume: 162
Category: Article
ISSN: 0378-1097
DOI: 10.1016/s0378-1097(98)00079-2

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An open reading frame (ORF) in the same operon as, but downstream of, vnfH in Azotobacter vinelandii can code for a ferredoxin-like protein. The role this ORF may play in the vnf (vanadium-dependent alternative) pathway of nitrogen fixation was investigated. Site-directed mutagenesis was used to alt