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Characterization of a Recombinant Murine 18.5-kDa Myelin Basic Protein

✍ Scribed by Ian R. Bates; Philip Matharu; Noboru Ishiyama; Denis Rochon; D.Denise Wood; Eugenia Polverini; Mario A. Moscarello; Nick J. Viner; George Harauz

Book ID
115645725
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
405 KB
Volume
20
Category
Article
ISSN
1046-5928

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Purification and spectroscopic character
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✍ Vladimir V. Bamm; Mumdooh A.M. Ahmed; Vladimir Ladizhansky; George Harauz πŸ“‚ Article πŸ“… 2007 πŸ› John Wiley and Sons 🌐 English βš– 691 KB

## Abstract A recombinant form of the murine Golli‐myelin basic protein (MBP) isoform BG21 (rmBG21) has been expressed in __E. coli__, and isolated to 96% purity via metal chelation chromatography. Characteristic yields were 6–8 mg protein per liter of culture in either minimal M9 or standard Luria