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Characterization of a Protein Conjugate Using an Asymmetrical-Flow Field-Flow Fractionation and a Size-Exclusion Chromatography with Multi-Detection System

✍ Scribed by Rebolj, Katja; Pahovnik, David; Žagar, Ema


Book ID
120397821
Publisher
American Chemical Society
Year
2012
Tongue
English
Weight
472 KB
Volume
84
Category
Article
ISSN
0003-2700

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Size-exclusion high-performance liquid chromatography (SE-HPLC, SEC) is the long-standing biopharmaceutical industry standard for quantitation of soluble protein aggregates. Recently, sedimentation velocity analytical ultracentrifugation (SV-AUC) has emerged as a possible orthogonal technique to SEC