Characterization of a mutation conferring radiation sensitivity, ior, located close to the gene coding for deoxycytidine deaminase in Escherichia coli
✍ Scribed by Estevenon, Anne-Marie ;Martin, Bernard ;Sicard, Nicole
- Publisher
- Springer
- Year
- 1985
- Tongue
- English
- Weight
- 545 KB
- Volume
- 200
- Category
- Article
- ISSN
- 0026-8925
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✦ Synopsis
The isolation and characterization of a new mutation conferring radiation sensitivity in Escherichia coli is described. This mutation is located close to the gene coding for deoxycytidine deaminase, in the chromosomal region of the gat operon. It is very sensitive to gamma rays and exhibits a decrease in recombination ability. The expression of radiation sensitivity seems to result from the additive effect of the dcd mutation and another mutation of unknown function.
Chemical reagents. N-Methyl-N-nitro-N-nitrosoguanidine (MNNG), and methyl methane sulphonate (MMS) were obtained from Aldrich Chemical Co., 5 bromodeoxyuridine (5 BrdU) was from Boehringer, 2'deoxycytidine 5' triphosphate was purchased from Sigma Chemical Co. and Biogel P6 was from Bio-Rad Laboratories.