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Characterization of a modified nitrogenase Fe protein fromKlebsiella pneumoniaein which the 4Fe4S cluster has been replaced by a 4Fe4Se cluster

✍ Scribed by Patrick Clark Hallenbeck; Graham N. George; Roger C. Prince; Roger N. F. Thorneley


Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
356 KB
Volume
14
Category
Article
ISSN
1432-1327

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We describe the insertion of an iron-sulfur center into a designed four alpha-helix model protein. The model protein was re-engineered by introducing four cysteine ligands required for the coordination of the mulinucleate cluster into positions in the main-chain directly analogous to the domain pred