Characterization of a gadolinium-labeled cholesterol derivative as an organ-specific contrast agent for adrenal MR imaging
✍ Scribed by Andreas Muhler; Johannes Platzek; Bernd Radüchel; Thomas Frenzel; Hanns-Joachim Weinmann
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 437 KB
- Volume
- 5
- Category
- Article
- ISSN
- 1053-1807
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✦ Synopsis
The purpose of the study was to determine if derivatization of cholesterol with a paramagnetic label could result in an organ-specific contrast agent for magnetic resonance imaging of the adrenal glands. Gadolinium-D03A-labeled cholesterol was synthesized and the relaxivities in water and blood plasma determined at 0.47 T and 40°C. Organ distribution was measured at 2 (n = 2) and 24 (n = 2) hours after intravenous injection of a 50 p.mol/kg dose of Gd-D03A-cholesterol in rats weighing 220-240 g. T1-weighted spin-echo images were acquired at 2 T before and after injection of 50 pmol/kg Gd-DO3A-cholesterol (n = 2) and GI-DTPA (diethylenetriaminepentaacetic acid)-albumin (n = 2). More than 99% of the Gd-DO3A-cholesterol was found to be protein bound in bovine serum. High T1 and T2 relaxivities were found in water and plasma. High tissue concentrations of Gd-DO3A-cholesterol were found only in adrenal glands and liver. At 24 hours, adrenal gadolinium concentrations were about 10 times higher than in blood. At 2 hours after injection of Gd-DO3A-cholesterol. enhancement was 162% in adrenal glands and 146% in liver. With Gd-DTPAalbumin, enhancement values were 57% and 56%. respectively. Index t e r n : Adrenal gland. MR. 86.12143 * Cholesterol -Contrast enhancement -Contrast media -Contrast medla. experlmental studles * Gadollnlum JMRl 1995: 5:7-LO Abbreviation% D03A-cholesterol = I IRS. ZSRI 10.1 1-(2.8-dioxa-8-[3b-(cholest-5-en)yl)-octyl)-2.3-dlhydroxypropylI-I .4.7-trlslcarboxymethyll-1.4.7.1 0-tetraazacyclodcdecane. DTPA = dlethylenetrlamlnepentaacellc acld.