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Characterization of a chitinase gene (chiA) from Serratia marcescens BJL200 and one-step purification of the gene product

✍ Scribed by May B. Brurberg; Vincent G.H. Eijsink; Ingolf F. Nes


Book ID
109320367
Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
438 KB
Volume
124
Category
Article
ISSN
0378-1097

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Serratia marcescens chitinase: One-step
✍ Rowena L. Roberts; Enrico Cabib πŸ“‚ Article πŸ“… 1982 πŸ› Elsevier Science 🌐 English βš– 849 KB

The extracellular chitinase produced by Serratia marcescens was obtained in highly purified form by adsorption-digestion on chitin. After gel electrophoresis in a nondenaturing system, the purified preparation exhibited two major protein bands that coincided with enzymatic activity. A study of the e