Characterization of a cDNA encoding cysteine proteinase inhibitor from Chinese cabbage (Brassica campestrisL. ssp.pekinensis) flower buds
✍ Scribed by Chae Oh Lim; Soo In Lee; Woo Sik Chung; Sung Han Park; Inhwan Hwang; Moo Je Cho
- Publisher
- Springer
- Year
- 1996
- Tongue
- English
- Weight
- 711 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0167-4412
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✦ Synopsis
A cDNA encoding a new phytocystatin isotype named BCPI-I was isolated from a cDNA library of Chinese cabbage flower buds. The BCPI-1 clone encodes 199 amino acids resulting in a protein much larger than other known phytocystatins. BCPI-1 has an unusually long C-terminus. A BCPI-1 fusion protein expressed in Escherichia coli strongly inhibits the enzymatic activity of papain, a cysteine proteinase. Genomic Southern blot analysis revealed that the BCPI gene is a member of a small multi-gene family in Chinese cabbage. Northern blot analysis showed that it is differentially expressed in the flower bud, leaf and root.