Characterization of a cDNA encoding a PSII-associated chlorophyll a/b-binding protein (CAB) fromChlamydomonas moewusiifitting into neither type I nor type II

✍ Scribed by Lucie Larouche; Colette Tremblay; Claire Simard; Guy Bellemare

• Book ID: 104737282
• Publisher: Springer-Verlag
• Year: 1991
• Tongue: English
• Weight: 333 KB
• Volume: 19
• Category: Article
• ISSN: 0172-8083
• DOI: 10.1007/bf00355056

✦ Synopsis

A full-length 1010-bp cDNA clone from ChIamydomonas moewusii coding for the precursor of a chlorophyll a/b-binding protein (CAB) was characterized. Northern analysis shows hybridization to a single 1 150-base light-stimulated mRNA. Complementary hybrid-selected mRNAs were translated in vitro; SDS-PAGE indicates the synthesis of three polypeptides of 25, 27 and 28 kDa. Comparison of the deduced polypeptide sequence with other published CABs reveals greater similarity with PSII-associated proteins but, as with other algal CABs, our sequence does not meet established criteria for inclusion into either type I or type II, so branching of CABs into two types seems to have occurred after the divergence between algae and land plants.