Characterization of a carbofuran-degrading bacterium and investigation of the role of plasmids in catabolism of the insecticide carbofuran

✍ Scribed by Ian M. Head; Ronald B. Cain; David L. Suett

• Book ID: 104680341
• Publisher: Springer
• Year: 1992
• Tongue: English
• Weight: 795 KB
• Volume: 158
• Category: Article
• ISSN: 0302-8933
• DOI: 10.1007/bf00245249

✦ Synopsis

A bacterium capable of using the carbamate insecticide carbofuran as a sole source of carbon and energy, was isolated from soil. The ability to catabolise carbofuran phenol, produced by cleavage of the carbamate ester linkage of the insecticide, was lost at very high frequency when the bacterium was grown in the absence of carbofuran. Plasmid analyses together with curing and mating experiments indicated that the presence of a large plasmid (pIH3, greater than 199 kb) was required for the degradation of carbofuran phenol.