✦ LIBER ✦

Characterization of a branched-chain amino-acid aminotransferase fromSchizosaccharomyces pombe

✍ Scribed by Eden, Amir; Benvenisty, Nissim

Publisher: John Wiley and Sons
Year: 1998
Tongue: English
Weight: 163 KB
Volume: 14
Category: Article
ISSN: 0749-503X
DOI: 10.1002/(sici)1097-0061(19980130)14:2<189::aid-yea210>3.0.co;2-v

No coin nor oath required. For personal study only.

✦ Synopsis

The Saccharomyces cerevisiae genes for the cytosolic and mitochondrial branched-chain amino-acid aminotransferases (BCAT) were isolated recently. These genes show significant homology to mammalian ECA39, originally isolated as a gene regulated by the c-myc oncogene. We now report the isolation of the Schizosaccharomyces pombe eca39/BCAT gene. The S. pombe protein shows 47-52% identity to other eukaryotic BCAT proteins isolated from S. cerevisiae, nematode, mouse and man. A genetic growth assay for BCAT activity was established using an S. cerevisiae strain disrupted in both BCAT isoenzymes. Consequently, the activity of the S. pombe BCAT was demonstrated by genetic and biochemical means. Possible applications of BCAT-encoding genes as selection markers in yeast transformation are proposed.

📜 SIMILAR VOLUMES

Enzymatic Method for Determination of Br

Enzymatic Method for Determination of Branched-Chain Amino Acid Aminotransferase Activity

✍ P. Schadewaldt; W. Hummel; U. Wendel; F. Adelmeyer 📂 Article 📅 1995 🏛 Elsevier Science 🌐 English ⚖ 519 KB

A method for branched-chain amino acid a

A method for branched-chain amino acid aminotransferase activity in microgram and nanogram tissue samples

✍ Carol S. Hintz; William R. Turk; Natalie Cambon; Helen B. Burch; Patti M. Nemeth 📂 Article 📅 1985 🏛 Elsevier Science 🌐 English ⚖ 399 KB

A method for branched-chain amino acid aminotransferase is described which is based on running the reaction in the reverse of the usual direction with glutamate and r\*-ketoisocaproate as substrates. The cY-ketoglutarate generated is reduced with glutamate dehydrogenase and NADH. For sensitivity in

Branched-chain amino acids and neurotran

Branched-chain amino acids and neurotransmitter metabolism: Expression of cytosolic branched-chain aminotransferase (BCATc) in the cerebellum and hippocampus

✍ Andrew J. Sweatt; Maria A. Garcia-Espinosa; Reidar Wallin; Susan M. Hutson 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 838 KB

Synthesis of α,β-Deuterated 15N Amino Ac

Synthesis of α,β-Deuterated 15N Amino Acids Using a Coupled Glutamate Dehydrogenase-Branched-Chain Amino Acid Aminotransferase System

✍ J.A. Chanatry; P.H. Schafer; M.S. Kim; D.M. Lemaster 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 480 KB

The Design and Synthesis of Human Branch

The Design and Synthesis of Human Branched-Chain Amino Acid Aminotransferase Inhibitors for Treatment of Neurodegenerative Diseases.

✍ Lain-Yen Hu; et al. et al. 📂 Article 📅 2006 🏛 John Wiley and Sons ⚖ 13 KB 👁 2 views

Is Intravenous Administration of Branche

Is Intravenous Administration of Branched Chain Amino Acids Effective in the Treatment of Hepatic Encephalopathy? A Multicenter Study

✍ John Wahren; Jacques Denis; Philippe Desurmont; Ljusk S. Eriksson; Jean-Marc Esc 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 701 KB

The influence of intravenous infusion of branched-chain amino acids (BCAAs) on brain function in patients with liver cirrhosis and acute hepatic encephalopathy was examined using a double-blind, randomized study design. Five medical centers in France and Sweden participated, and 50 patients were stu