Characterization by T1-oligonucleotide fingerprinting of three strains of human hepatitis A virus isolated in Italy

✍ Scribed by L. Fiore; E. Paolis; F. Novello; M. Divizia; A. Panà

• Book ID: 104646561
• Publisher: Springer
• Year: 1990
• Tongue: English
• Weight: 906 KB
• Volume: 6
• Category: Article
• ISSN: 0393-2990
• DOI: 10.1007/bf00155545

✦ Synopsis

Three human hepatitis A virus strains, all of them isolated in Italy but one acquired abroad, were analyzed by T1 -RNAase oligonucleotide mapping and by monoclonal antibody neutralization. The variation among their genomes according to T~ -maps was calculated to be about 9%, thus confirming the poor genomic variation assessed by nucleotide sequencing (1 -10%). However T~maps of these Italian isolates were different from those reported in the literature (Weitz and Siegl, 1985). Neutralization by monoclonal antibody caused a reduction in titres of 2 -2.5 lOgl0.

This genomic stability, if confirmed, is important with a view to a valuable vaccine.