Characterization and screening of microsatellite loci in a wild lemur population (Propithecus verreauxi verreauxi)

✍ Scribed by Richard R. Lawler; Alison F. Richard; Margaret A. Riley

• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 60 KB
• Volume: 55
• Category: Article
• ISSN: 0275-2565
• DOI: 10.1002/ajp.1058

✦ Synopsis

Abstract

Sixteen dinucleotide microsatellite loci were isolated from the genome of Propithecus verreauxi verreauxi. All loci were polymorphic when genotyped on a minimum of 16 animals. The number of alleles across these loci ranges from two to 11. Additionally, seven of these loci were genotyped across a minimum of 200 animals in order to estimate heterozygosity and their potential for parentage assignment in this population. Using these seven loci, the mean heterozygosity in this population is 0.705, and the combined probability of these seven loci to exclude a random individual from parentage, when one parent is known, is 0.996. These data suggest that these loci will be useful for estimating a variety of population genetic and genealogical parameters in P. v. verreauxi populations. Am. J. Primatol. 55:253–259, 2001. © 2001 Wiley‐Liss, Inc.