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Characterization and regeneration of wheat (Triticum aestivumL.) embryogenic cell suspension cultures

✍ Scribed by Flona A. Redway; Vimla Vasil; Indra K. Vasil

Publisher: Springer
Year: 1990
Tongue: English
Weight: 727 KB
Volume: 8
Category: Article
ISSN: 0721-7714
DOI: 10.1007/bf00272101

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✦ Synopsis

Stable cell suspension cultures were established from two types of calli (one compact, nodular and embryogenic, the other friable and embryogenic) derived from cultured immature embryos of wheat (cv FLA302). Only aged calli, which had been subcultured for at least 5-8 months, formed suspensions comprised mainly of groups of small, round, densely cytoplasmic, starch-containing cells. Only the embryogenic suspension derived from the aged, compact and nodular callus formed distinct somatic embryos when plated on regeneration media containing IAA and zeatin. Upon subsequent transfer to fresh regeneration medium more than 200 green rooted plants were obtained.

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