Characterization and optimization of β-galactosidase immobilization process on a mixed-matrix membrane
✍ Scribed by Peter Jochems; Yamini Satyawali; Sandra Van Roy; Wim Doyen; Ludo Diels; Winnie Dejonghe
- Book ID
- 113606162
- Publisher
- Elsevier Science
- Year
- 2011
- Tongue
- English
- Weight
- 536 KB
- Volume
- 49
- Category
- Article
- ISSN
- 0141-0229
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## Abstract Many alternative strategies to immobilize and stabilize enzymes have been investigated in recent years for applications in biosensors. The entrapment of enzymes within silica‐based nanospheres formed through silicification reactions provides high loading capacities for enzyme immobiliza
The activity of b-galactosidase, immobilized by grafting technique on Teflon membranes preactivated with four different monomers, has been characterized from the biochemical and biophysical points of view. The monomers used were acrylic acid or acrylamide, or methacrylic acid and 2-hydroxyethyl meth