Characterization and molecular cloning of thermostable alpha-amylase fromStreptomycessp.To1

✍ Scribed by Lotfi Mellouli; Raoudha Ghorbel; Alya Kammoun; Monia Mezghani; Samir Bejar

• Publisher: Springer Netherlands
• Year: 1996
• Tongue: English
• Weight: 353 KB
• Volume: 18
• Category: Article
• ISSN: 0141-5492
• DOI: 10.1007/bf00127894

✦ Synopsis

A new thermophilic Streptomyces sp. TO1, isolated from Tunisian soil, produced a thermostable alpha-amylase and pullulanase. The gene encoding for the alpha-amylase activity was cloned into the multicopy cloning plasmid pLM1 using S. lividans ZX1 as host strain. The ZX1 / pLM1 strain has the same activity than the initial TO1 strain and about 25 fold higher activity than the ZX1 strain. This alpha-amylase has an optimum of pH and temperature at 6 and 700C respectively.